To explore the response of N2O emissions from greenhouse soil to inoculated plant growth-promoting rhizobacteria (PGPR), four PGPR strains, including Bacillus subtilis subsp. subtilis NRCB002, Stutzerimonas stutzeri NRCB010, Bacillus velezensis NRCB026, and Achromobacter denitrificans YSQ030, were used as test strains. Tomato was used as the test crop, and randomized block design experiments were conducted under greenhouse conditions. The results showed that even under moderately saline stress soil conditions, PGPR inoculation treatment exhibited N2O emission reduction tendency in the first season compared with the CK treatment. In the second season, the treatment with YSQ030 inoculation showed a significant emission reduction effect, reducing soil N2O cumulative emissions by 36%. The four strains of PGPR demonstrated certain promoting effects on the growth and yield of tomato plants. The research results indicated that PGPR played an important role in reducing N2O emissions from greenhouse soil and promoting crop growth.