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接种氧化亚氮(N2O)还原细菌YSQ030对复垦土壤N2O排放和氮循环关键功能基因的影响
作者:
作者单位:

1.南京信息工程大学;2.南京工业大学生物与制药工程学院/国家生化工程技术研究中心;3.中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室

基金项目:

国家自然科学(31972503和41771291)和江苏省重点研发计划(社会发展)项目(BE2020731)。


Effects of inoculation with nitrous oxide (N2O)-reducing bacteria YSQ030 on soil N2O emission and key functional genes involved in nitrogen cycling in reclaimed soilZHU Jinhong1, XIONG Ruonan1, YANG Siqi1, GAO Nan2, WU Yonghong3, SHEN Weishou1
Author:
Affiliation:

1.Nanjing University of Information Science &2.Technology;3.College of Biotechnology and Pharmaceutical Engineering / National Engineering Research Center for Biotechnology, Nanjing Tech University;4.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences

Fund Project:

National Natural Science Foundation of China (31972503, 41771291) and Key Research and Development Plan Program of Jiangsu Province (BE2020731).

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    摘要:

    复垦土地是重要的后备土地资源,但通常土壤结构差、有机质和养分含量低；增施有机肥是快速提升地力的关键途径,但会造成温室气体如氧化亚氮(N2O)的大量排放。接种具有N2O还原功能的植物根际促生菌(PGPR)不仅能够减少温室气体排放,还能促进作物生长。本研究以一株具有N2O还原功能的PGPR反硝化无色杆菌(Achromobacter denitrificans YSQ030)为供试菌株,明确接种YSQ030对施用有机肥的复垦土壤N2O排放和氮循环关键功能基因的影响。通过设置施用商品有机肥和羊粪有机肥的土壤微宇宙试验,利用气相色谱仪分析接种YSQ030后土壤N2O排放通量,进一步计算累积排放量；在试验结束后分析土壤pH、EC、硝态氮和铵态氮含量,并利用实时荧光定量PRC分析土壤硝化功能基因(AOA amoA和AOB amoA)和反硝化功能基因(nirS、nirK、nosZ I和nosZ II)的丰度。结果显示,施用商品有机肥和羊粪有机肥的土壤中接种YSQ030明显减少复垦土壤N2O排放,减少N2O排放量最大分别达90％和30％。施用商品有机肥处理的N2O排放量远高于施用羊粪有机肥处理,这可能是由于施用商品有机肥的土壤中编码反硝化细菌N2O还原酶基因nosZ I 和非典型反硝化细菌N2O还原酶基因nosZ II基因丰度,相对于施用羊粪有机肥的土壤明显较低。施用商品有机肥均显著降低土壤硝化和反硝化功能基因丰度,而施用羊粪有机肥对土壤硝化和反硝化功能基因丰度大多没有明显影响。本研究表明,接种YSQ030能够减少施用有机肥土壤的N2O排放,将为复垦土壤地力提升和N2O减排提供科学依据,也将为研发新型微生物肥料或生物有机肥提供核心菌种资源。

    Abstract:

    Reclaimed land is an important reserve land resource, but usually the soil structure is poor, and the organic matter and nutrient content are low. Increasing the application of organic fertilizers is a key way to quickly improve soil productivity, but it will cause large emissions of greenhouse gases such as nitrous oxide (N2O). Inoculation with plant rhizosphere growth promoters (PGPR) with N2O reduction function not only reduces greenhouse gas emissions, but also promotes crop growth. In this study, a PGPR denitrificans YSQ030 with N2O reduction function was used as the test strain to clarify the effect of inoculation YSQ030 on N2O emission and nitrogen cycling key functional genes in reclaimed soil with organic fertilizer application. By setting up soil microcosmic experiments for the application of commercial organic fertilizer and sheep manure organic fertilizer, the soil N2O emission flux after inoculation YSQ030 was analyzed by gas chromatography, and the cumulative emission was further calculated. Soil pH, EC, nitrate nitrogen and ammonium nitrogen content were analyzed at the end of the experiment, and the abundance of soil nitrification functional genes (AOA amoA and AOB amoA) and denitrification functional genes (nirS, nirK, nosZ I and nosZ II) was analyzed by real-time real-time quantitative PRC. The results showed that inoculation of YSQ030 in soil applied to commercial organic fertilizer and sheep manure organic fertilizer significantly reduced the N2O emission of reclaimed soil, and the maximum reduction of N2O emission was 90% and 30%, respectively. The N2O emissions of commercial organic fertilizer treatment were much higher than those of sheep manure organic fertilizer treatment, which may be due to the fact that the abundance of the N2O reductase gene nosZ I and the nosZ II gene of the N2O reductase gene of atypical denitrifying bacteria N2O reductase in the soil applied to commercial organic fertilizer was significantly lower than that of the soil where sheep manure organic fertilizer was applied. The application of commercial organic fertilizer significantly reduced the abundance of functional genes of soil nitrification and denitrification, while the application of sheep manure organic fertilizer had no obvious effect on the abundance of functional genes of soil nitrification and denitrification. This study shows that inoculation with YSQ030 can reduce the N2O emission in soil applied with organic fertilizer, which will provide a scientific basis for soil fertility improvement and N2O emission reduction, and will also provide core strain resources for the research and development of new microbial fertilizers or bio-organic fertilizers.

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朱津宏,熊若男,杨思琪,高南,吴永红,申卫收.接种氧化亚氮(N2O)还原细菌YSQ030对复垦土壤N2O排放和氮循环关键功能基因的影响[J].南京信息工程大学学报,,():

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  • 收稿日期:2023-03-12
  • 最后修改日期:2023-05-02
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