Porous TiO₂ microspheres with nanocrystalline have the advantages of big grain size,small crystalline size and high specific surface.In this study,porous TiO₂ microspheres with nanocrystalline were fabricated by using butyl titanate as raw material and gelatin as a template with the sol-gel method.Then their properties were characterized by transmission electron microscope and X-ray diffraction.The results showed that the pure anatase TiO₂ was gained and its composite was not changed when the gelatin solution was added.However,the gelatin refined and rearranged TiO₂ crystallizes.It was demonstrated that porous TiO₂ microspheres with the diameter of about 200-500 nm were formed,and consisted of nanocrystallizes with the average size of(14.3±0.9)nm and pores about 2-5 nm.Moreover,the formation mechanism of porous TiO₂ microspheres was analyzed.